PRIMER_FIRST_BASE_INDEX=1
PRIMER_GC_CLAMP=0
PRIMER_INTERNAL_MAX_NS_ACCEPTED=0
PRIMER_INTERNAL_MAX_POLY_X=5
PRIMER_INTERNAL_MAX_SIZE=27
PRIMER_INTERNAL_MIN_3_PRIME_OVERLAP_OF_JUNCTION=3
PRIMER_INTERNAL_MIN_5_PRIME_OVERLAP_OF_JUNCTION=3
PRIMER_INTERNAL_MIN_QUALITY=0
PRIMER_INTERNAL_MIN_SIZE=18
PRIMER_INTERNAL_MIN_THREE_PRIME_DISTANCE=-1
PRIMER_INTERNAL_OPT_SIZE=20
PRIMER_LIBERAL_BASE=0
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
PRIMER_LOWERCASE_MASKING=0
PRIMER_MAX_END_GC=5
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MAX_POLY_X=5
PRIMER_MAX_SIZE=35
PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION=4
PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION=7
PRIMER_MIN_END_QUALITY=0
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=-1
PRIMER_MIN_QUALITY=0
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=-1
PRIMER_MIN_SIZE=30
PRIMER_NUM_RETURN=50
PRIMER_OPT_SIZE=33
PRIMER_PICK_ANYWAY=0
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_QUALITY_RANGE_MAX=100
PRIMER_QUALITY_RANGE_MIN=0
PRIMER_SECONDARY_STRUCTURE_ALIGNMENT=0
PRIMER_SEQUENCING_ACCURACY=20
PRIMER_SEQUENCING_INTERVAL=250
PRIMER_SEQUENCING_LEAD=50
PRIMER_SEQUENCING_SPACING=500
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=0
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0
SEQUENCE_FORCE_LEFT_END=-1000000
SEQUENCE_FORCE_LEFT_START=-1000000
SEQUENCE_FORCE_RIGHT_END=-1000000
SEQUENCE_FORCE_RIGHT_START=-1000000
SEQUENCE_START_CODON_POSITION=-2000000
PRIMER_ANNEALING_TEMP=-10
PRIMER_DMSO_CONC=0
PRIMER_DMSO_FACTOR=0.6
PRIMER_DNA_CONC=50
PRIMER_DNTP_CONC=0
PRIMER_FORMAMIDE_CONC=0
PRIMER_INSIDE_PENALTY=-1
PRIMER_INTERNAL_DMSO_CONC=0
PRIMER_INTERNAL_DMSO_FACTOR=0.6
PRIMER_INTERNAL_DNA_CONC=50
PRIMER_INTERNAL_DNTP_CONC=0
PRIMER_INTERNAL_FORMAMIDE_CONC=0
PRIMER_INTERNAL_MAX_BOUND=110
PRIMER_INTERNAL_MAX_GC=80
PRIMER_INTERNAL_MAX_HAIRPIN_TH=47
PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12
PRIMER_INTERNAL_MAX_SELF_ANY=12
PRIMER_INTERNAL_MAX_SELF_ANY_TH=47
PRIMER_INTERNAL_MAX_SELF_END=12
PRIMER_INTERNAL_MAX_SELF_END_TH=47
PRIMER_INTERNAL_MAX_TM=63
PRIMER_INTERNAL_MIN_BOUND=-10
PRIMER_INTERNAL_MIN_GC=20
PRIMER_INTERNAL_MIN_TM=57
PRIMER_INTERNAL_OPT_BOUND=97
PRIMER_INTERNAL_OPT_TM=60
PRIMER_INTERNAL_SALT_DIVALENT=0
PRIMER_INTERNAL_SALT_MONOVALENT=50
PRIMER_INTERNAL_WT_BOUND_GT=0
PRIMER_INTERNAL_WT_BOUND_LT=0
PRIMER_INTERNAL_WT_END_QUAL=0
PRIMER_INTERNAL_WT_GC_PERCENT_GT=0
PRIMER_INTERNAL_WT_GC_PERCENT_LT=0
PRIMER_INTERNAL_WT_HAIRPIN_TH=0
PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0
PRIMER_INTERNAL_WT_NUM_NS=0
PRIMER_INTERNAL_WT_SELF_ANY=0
PRIMER_INTERNAL_WT_SELF_ANY_TH=0
PRIMER_INTERNAL_WT_SELF_END=0
PRIMER_INTERNAL_WT_SELF_END_TH=0
PRIMER_INTERNAL_WT_SEQ_QUAL=0
PRIMER_INTERNAL_WT_SIZE_GT=1
PRIMER_INTERNAL_WT_SIZE_LT=1
PRIMER_INTERNAL_WT_TM_GT=1
PRIMER_INTERNAL_WT_TM_LT=1
PRIMER_MAX_BOUND=110
PRIMER_MAX_END_STABILITY=100
PRIMER_MAX_GC=80
PRIMER_MAX_HAIRPIN_TH=47
PRIMER_MAX_LIBRARY_MISPRIMING=12
PRIMER_MAX_SELF_ANY=5
PRIMER_MAX_SELF_ANY_TH=47
PRIMER_MAX_SELF_END=3
PRIMER_MAX_SELF_END_TH=47
PRIMER_MAX_TEMPLATE_MISPRIMING=-100
PRIMER_MAX_TEMPLATE_MISPRIMING_TH=-100
PRIMER_MAX_TM=80
PRIMER_MIN_BOUND=-10
PRIMER_MIN_GC=20
PRIMER_MIN_TM=20
PRIMER_OPT_BOUND=97
PRIMER_OPT_TM=55
PRIMER_OUTSIDE_PENALTY=0
PRIMER_PAIR_MAX_COMPL_ANY=5
PRIMER_PAIR_MAX_COMPL_ANY_TH=47
PRIMER_PAIR_MAX_COMPL_END=3
PRIMER_PAIR_MAX_COMPL_END_TH=47
PRIMER_PAIR_MAX_DIFF_TM=100
PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=24
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=-100
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH=-100
PRIMER_PAIR_WT_COMPL_ANY=0
PRIMER_PAIR_WT_COMPL_ANY_TH=0
PRIMER_PAIR_WT_COMPL_END=0
PRIMER_PAIR_WT_COMPL_END_TH=0
PRIMER_PAIR_WT_DIFF_TM=0
PRIMER_PAIR_WT_IO_PENALTY=0
PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0
PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0
PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0
PRIMER_PAIR_WT_PRODUCT_TM_GT=0
PRIMER_PAIR_WT_PRODUCT_TM_LT=0
PRIMER_PAIR_WT_PR_PENALTY=1
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH=0
PRIMER_PRODUCT_MAX_TM=1000000
PRIMER_PRODUCT_MIN_TM=-1000000
PRIMER_SALT_DIVALENT=0
PRIMER_SALT_MONOVALENT=50
PRIMER_WT_BOUND_GT=0
PRIMER_WT_BOUND_LT=0
PRIMER_WT_END_QUAL=0
PRIMER_WT_END_STABILITY=0
PRIMER_WT_GC_PERCENT_GT=0
PRIMER_WT_GC_PERCENT_LT=0
PRIMER_WT_HAIRPIN_TH=0
PRIMER_WT_LIBRARY_MISPRIMING=0
PRIMER_WT_MASK_FAILURE_RATE=0
PRIMER_WT_NUM_NS=0
PRIMER_WT_POS_PENALTY=1
PRIMER_WT_SELF_ANY=0
PRIMER_WT_SELF_ANY_TH=0
PRIMER_WT_SELF_END=0
PRIMER_WT_SELF_END_TH=0
PRIMER_WT_SEQ_QUAL=0
PRIMER_WT_SIZE_GT=1
PRIMER_WT_SIZE_LT=1
PRIMER_WT_TEMPLATE_MISPRIMING=0
PRIMER_WT_TEMPLATE_MISPRIMING_TH=0
PRIMER_WT_TM_GT=1
PRIMER_WT_TM_LT=1
SEQUENCE_PRIMER=
SEQUENCE_INTERNAL_OLIGO=
SEQUENCE_PRIMER_REVCOMP=
SEQUENCE_OVERHANG_LEFT=
SEQUENCE_OVERHANG_RIGHT=
SEQUENCE_TARGET=
SEQUENCE_OVERLAP_JUNCTION_LIST=
SEQUENCE_INTERNAL_OVERLAP_JUNCTION_LIST=
SEQUENCE_EXCLUDED_REGION=
SEQUENCE_PRIMER_PAIR_OK_REGION_LIST=
SEQUENCE_INCLUDED_REGION=
SEQUENCE_INTERNAL_EXCLUDED_REGION=
SEQUENCE_START_CODON_SEQUENCE=ATG
PRIMER_MUST_MATCH_FIVE_PRIME=
PRIMER_MUST_MATCH_THREE_PRIME=
PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME=
PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME=
PRIMER_PRODUCT_SIZE_RANGE=200-500
SEQUENCE_QUALITY=
PRIMER_TASK=generic
PRIMER_TM_FORMULA=0
PRIMER_SALT_CORRECTIONS=0
PRIMER_MISPRIMING_LIBRARY=
PRIMER_INTERNAL_MISHYB_LIBRARY=
=
